Fig. 2: IL-33/ST2 is required for chronic pain in both male and female mice in the CFA model.
From: IL-33/ST2 drives inflammatory pain via CCL2 signaling and activation of TRPV1 and TRPM8
Mechanical allodynia (A) and thermal hyperalgesia (B) were attenuated in both IL33−/− and ST2−/− male mice at day 3 after CFA injection (n = 6 mice). Mechanical allodynia (C) and thermal hyperalgesia (D) were attenuated in both IL33−/− and ST2−/− male mice at day 9 after CFA injection (n = 6 mice). Mechanical allodynia (E) and thermal hyperalgesia (F) were partially reversed in the ipsilateral hindpaw of IL-33−/− female mice at day 3 after CFA injection (n = 6 mice). Mechanical allodynia (G) and thermal hyperalgesia (H) were partially reversed in the ipsilateral hindpaw of ST2−/− female mice at day 3 after CFA injection (n = 6 mice). Mechanical allodynia (I) and thermal hyperalgesia (J) were partially reversed in ST2−/− female mice at day 9 after CFA injection (n = 6 mice). K Mechanical allodynia was partially attenuated in ST2−/− male mice in the MIA model (n = 6 mice). Data are presented as mean ± SEM. **p < 0.01, ****p < 0.0001, #p < 0.05, ###p < 0.001, ####p < 0.0001, p < 0.0001. *represents PBS vs CFA or MIA in WT mice. #represents WT vs IL-33−/− mice in (A–F) or ST2−/− in (G–J) for the CFA model and (K) in the MIA model, represents WT vs ST2−/− mice in the CFA model in (A–D). One-way ANOVA with Bonferroni’s post-hoc test for (A–J); Repeated measures for Two-way ANOVA with Bonferroni’s post-hoc test for (K); CFA complete Freund’s adjuvant, MIA mono-iodoacetate.
