Fig. 1: Lung cancer induced by TAT-CRE and AD-CRE showed corresponding macroscopic tumor growth parameters.
Lung adenocarcinomas driven by a CRE-inducible conditional KrasG12D mutant and functional Trp53 knock out were induced by TAT-CRE (n = 5, 3 males, 2 females) and AD-CRE inhalation (n = 5, 3 males, 2 females) and macroscopically analyzed. a Probability of survival was analyzed as Kaplan-Meier-Curves and statistically evaluated using the Mantel-Cox test. b Picture of final stage lung adenocarcinomas indicating the target lesion (white), the non-target lesions (black) and the heart (H). Bars indicate 8 mm. c Lung weight was determined directly after harvest of the lungs. d Body weight at T0 (3 weeks after inhalation) and TF (final stage before lung harvest). e, f Tumor onset and target lesion diameter at T0 (3 weeks after inhalation) and TF (final stage before lung harvest) lesions were determined based on µCT DICOM data analysis using OsiriX. c-f p-values were calculated using an unpaired two-sided t-test. Data is shown as mean ± SD. P-values ≤ 0.05 are considered as significant. g Representative weekly µCT measurements starting 3 weeks after CRE inhalation. Target lesion and heart (H) are indicated. h, i Tumor volume and non-target lesions were determined based on µCT DICOM data analysis using OsiriX. Statistical analysis was done using ANOVA. P-values ≤ 0.05 are considered as significant.
