Fig. 4: TAT-CRE induced lung tumors showed significantly increased micro-vessels compared to AD-CRE induced lung tumors. | Communications Biology

Fig. 4: TAT-CRE induced lung tumors showed significantly increased micro-vessels compared to AD-CRE induced lung tumors.

From: TAT-CRE inhalation enables tumor induction corresponding to adenoviral Cre-recombinase in a lung cancer mouse model

Fig. 4

Lung adenocarcinomas induced by TAT-CRE (n = 4, 2 males, 2 females) and AD-CRE (n = 4, 2 males, 2 females) inhalation were analyzed regarding cell proliferation, micro-vessel density and apoptosis based on IHC stains. a KI-67 stain was analyzed to determine the cell proliferation rate, taking into account the average of all tumor lesions per slice. b Cleaved Caspase 3 stain was analyzed to determine the apoptosis rate, taking into account the average of all tumor lesions per slice. c CD31 stain was analyzed to determine the micro-vessel density per field of view (FOV) of 200 µm². d Representative IHC stains of KI-67, CD31, cleaved Caspase-3 and H&E, magnification 20x. Bars indicate 100 µm. p-values were calculated using an unpaired two-sided t-test. Data is shown as mean ± SD. P-values ≤ 0.05 are considered as significant.

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