Fig. 4: Interference of LAPTM4A Affects M1/M2 Phenotype Switch in Glioblastoma TAMs. | Communications Biology

Fig. 4: Interference of LAPTM4A Affects M1/M2 Phenotype Switch in Glioblastoma TAMs.

From: Loss of LAPTM4A inhibits M2 polarization of tumor-associated macrophages in glioblastoma, promoting immune activation and enhancing anti-PD1 therapy

Fig. 4

A Induction of TAMs by co-culturing T98G or LN229 glioblastoma cells with THP-1 cells infected with or without sh-NC/shLAPTM4A; B RT-qPCR analysis of the expression levels of M2 (CD163, IL-10, Arg-1) and M1 (CD86, iNOS, TNF-α) macrophage markers in macrophages co-cultured with T98G glioblastoma cells; C Detection of TNF-α and IL-10 production by ELISA; D, E Immunofluorescence staining of CD86 and CD206 expression in macrophages, with green fluorescence labeling CD86 or CD206, blue fluorescence labeling DAPI (Scale Bar = 25 μm); F Western blot analysis of CD86 and MRC-1 expression. *P < 0.05, cell experiments repeated at least three times.

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