Fig. 7: Influence of LAPTM4A on the polarization of M2 macrophages in Glioma. | Communications Biology

Fig. 7: Influence of LAPTM4A on the polarization of M2 macrophages in Glioma.

From: Loss of LAPTM4A inhibits M2 polarization of tumor-associated macrophages in glioblastoma, promoting immune activation and enhancing anti-PD1 therapy

Fig. 7

A RT-qPCR detection of M1 (CD86, iNOS, and TNF-α) and M2 (CD163, IL-10, Arg-1) macrophage marker expression levels in glioma model mice; B ELISA detection of TNF-α and IL-10 production; C Flow cytometry detection of CD86 and CD206 expression; D Western blot detection of CD86 and MRC-1 expression; E Representative images of Iba1 (red), LAPTM4A (white), and GL261-GFP (green) in glioma models, as well as merged images of WT (top) and LAPTM4A-KO mice (bottom) (Scale Bar = 25 μm); F Quantitative determination of the percentage of Iba1+ coverage area in Glioma (GL261-GFP); G Quantitative determination of the LAPTM4A+ coverage area inside and outside Glioma (normalized to Iba1+ coverage area). n = 10, *P < 0.05.

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