Fig. 4: Genes related to mitochondrial properties show transcriptomic alterations in neuronal models of aging.
a The mRNA expression profile of aged iNs and aged iPSCsNs % normalized to the corresponding young. The smaller graph represented the mRNA expression of the genes FOXO1 and UCP2, which had the highest percentage difference compared to the corresponding young (N = 3 independent experiments, n = 3 replicates per experiment). b Aged iNs versus aged iPSCsNs of detected genes in our investigation. Principal component analysis of aged iNs (red) and aged iPSCsNs (green). Scores plot between the selected PC1 and PC2. Each collared dot represents a replicate (N = 3 independent experiments, n = 3 replicates per experiment). Data information: The represented values for the gene expression were assessed from three donors with three technical replicates for each condition. Student’s unpaired t-test was performed to compare the gene expression between young and aged iNs and between young and aged iPSCsNs. Therefore, the asterisks (*p < 0.05, **p < 0.01, ***p < 0.001) indicate a statistically significant difference between aged iNs vs. young iNs or young iPSCsNs vs. aged iPSCsNs, analyzing the same neuronal models between young and aged groups (*p < 0.05, **p < 0.01, ***p < 0.001). The detailed statistical parameters, including the number of values, minimum, maximum, range, mean, standard deviation, and standard error of the mean, are presented in Supplementary Data 2. b was generated on metaboanalyst.ca. The represented values were normalized by autoscaling (mean-centered and divided by the standard deviation of each variable). The data were represented as Gene expression (2(−Avg.(Delta(Ct))) by using the housekeeping gene GAPDH, which is calculated by the website https://geneglobe.qiagen.com/us/analyze. b was generated using metaboanalyst.ca.
