Fig. 8: Representative z-projection microscopy images of the mitochondrial network stained with the TOMM20 antibody in young HFs (×63 magnification). | Communications Biology

Fig. 8: Representative z-projection microscopy images of the mitochondrial network stained with the TOMM20 antibody in young HFs (×63 magnification).

From: Tracing mitochondrial marks of neuronal aging in iPSCs-derived neurons and directly converted neurons

Fig. 8

The left panel shows the raw image after background subtraction (rolling ball = 5 pixels). The middle panel (“tubeness”) displays the same images processed with the morphometry macro in FIJI software, as mentioned in ref. 112. The right panel (“skeletonized”) presents the binary representations of the mitochondrial network (in black) after further image processing using FIJI’s “Skeletonize” function.

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