Table 3 Mitochondrial stress test parameters

From: Tracing mitochondrial marks of neuronal aging in iPSCs-derived neurons and directly converted neurons

Assay parameter

Equation

Definition

Non-Mitochondrial Oxygen Consumption

Minimum rate after rotenone/antimycin A injection

OCR from non-mitochondrial sources; essential for accurate mitochondrial measurements.

Basal Respiration

(Mean measurements before first injection)−(Non-Mitochondrial Oxygen Consumption)

Baseline oxygen consumption for ATP production via mitochondria.

ATP-Production Coupled Respiration

(Basal Respiration)−(Proton Leak)

Portion of basal respiration used for ATP synthesis; indicated by OCR drop after oligomycin.

Maximal Respiration

(Maximum rate after FCCP injection)−(Non-Mitochondrial Oxygen Consumption)

Highest achievable OCR after FCCP uncoupling.

Spare Respiratory Capacity

(Maximal Respiration)−(Basal Respiration)

Difference between maximal and basal respiration; indicates the cell’s ability to meet increased energy demands.

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