Table 4 Glycolysis stress test parameters

From: Tracing mitochondrial marks of neuronal aging in iPSCs-derived neurons and directly converted neurons

Assay parameter

Equation

Definition

Non-Glycolytic Acidification

Minimum rate measurement after 2-DG injection

ECAR from non-glycolytic sources; essential for accurate glycolysis detection.

Basal glycolysis or

glycolysis

(Maximum rate measurement between glucose and oligomycin injection)−(Non-Glycolytic Acidification)

Baseline glycolysis rate after glucose addition.

Glycolytic Capacity

(Maximum rate measurement between Oligomycin and 2-DG injection)−(Non-Glycolytic Acidification)

Maximum glycolysis rate when OXPHOS is inhibited, forcing cells to rely entirely on glycolysis.

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