Fig. 5: STDP is determined by the degree of NMDA receptor-driven membrane depolarization.
From: Coincidence detection between apical and basal dendrites drives STDP in cerebellar Golgi cells
A Sample traces (average of 60 sweeps) elicited during STDP induction showing AUC measured in response to mf-EPSP → pf-AP (purple) or pf-AP → mf-EPSP (orange) pairing. A similar experiment was repeated in the presence of NMDA receptor antagonists APV and 7-Cl-Kyn. The bar graphs show the average AUC value changes for Δt = −25 ms (orange) and Δt = +25 ms (purple) in control and with NMDA receptor antagonists (pf-AP → mf-EPSP: n = 5, N = 5, vs., n = 11, N = 10, p = 0.013; mf-EPSP → pf-AP: n = 5, N = 4, vs. n = 11, N = 10, p = 0.0005). B The plot shows the correlation between EPSC amplitude changes and the corresponding AUC value for each GoC recorded at Δt = −25 ms (large orange dots) and Δt = +25 ms (large purple dots), with individual recordings overlaid (small dots). Dotted line represents the linear fit to data points (Adj R2 = 0.44, n = 22, N = 20, p = 4.66*10-4). C Sample traces obtained as the difference between those measured in control condition and those obtained with NMDA receptor antagonists for Δt = −25 ms (orange) and Δt = +25 ms (purple). The bar graph shows a significantly smaller AUCNMDA at Δt = −25 ms (orange) than at Δt = +25 ms (purple; n = 5, N = 5, vs. n = 5, N = 5, p = 0.008). **, p < 0.01, ***, p < 0.001; Student’s unpaired t test.
