Fig. 2: bishu-1 functioned in cool temperature avoidance in DOCCs. | Communications Biology

Fig. 2: bishu-1 functioned in cool temperature avoidance in DOCCs.

From: Monoacylglycerol acyltransferase maintains ionotropic receptor expression for cool temperature sensing and avoidance in Drosophila

Fig. 2

Preference indices (PIs) of two-way choice assays in a 20 °C versus 24 °C condition with the indicated genotypes. A, B Knockdown of bishu-1 (A) or bishu-2 (B) in IR25a-expressing neurons (N = 5–9 trials). C, D Rescue of bishu-1KO (C) or bishu-2KO (D) by introducing a wild-type transgene under the control of IR25a-GAL4 (N = 5–7 trials). E, F Knockdown of bishu-1 or bishu-2 in DOCCs using a specific driver IR21a-GAL4 (E) or R11F02-GAL4 (F, N = 5–8 trials). G Rescue of bishu-1KO by overexpressing wild-type bishu-1 in DOCCs using R11F02-GAL4 (N = 5–6 trials). H Thermal choice of bishu-1KO overexpressing human genes. Transgenes of human MGAT2 (UAS-hMGAT2), MGAT3 (UAS-hMGAT3), and DGAT2 (UAS-hDGAT2) were expressed in bishu-1KO using R11F02-GAL4 (N = 5–7 trials). The data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 by one-way ANOVA with Dunnett’s test (A–C and E–H) or the Kruskal–Wallis test with Steel’s test (D). NS denotes not significant.

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