Fig. 3: Correlation between viral genome copies/µL and mean percentage genome coverage across different sample types (Vero E6 propagates, CSF, urine, and phlebotomine pools). | Communications Biology

Fig. 3: Correlation between viral genome copies/µL and mean percentage genome coverage across different sample types (Vero E6 propagates, CSF, urine, and phlebotomine pools).

From: Development of a novel amplicon based whole-genome sequencing framework for improved surveillance of Toscana virus

Fig. 3

The size of the circles represents sequencing depth, highlighting the relationship between viral load (indicated by copies/µL), sequencing performance (mean genome coverage), and consensus sequence reliability (depth). Higher viral loads are associated with higher coverage and sequencing depth, with Vero E6 samples consistently achieving near-complete coverage. Variability is observed in clinical (CSF and urine) and vector (phlebotomine pool) samples, reflecting differences in matrix complexity and RNA quality, leading to different sequencing outputs. Vero E6 propagates are represented in green, CSF samples are represented in purple, urine samples are represented in pink, and phlebotomine are represented in orange.

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