Fig. 4: AP-1 factor motifs are enriched in primed chromatin regions in response to matrix stiffness. | Communications Biology

Fig. 4: AP-1 factor motifs are enriched in primed chromatin regions in response to matrix stiffness.

From: Increased matrix stiffness promotes fibrogenesis of hepatic stellate cells through AP-1-induced chromatin priming

Fig. 4

a Transcription factor enrichment in primed chromatin regions. b ATAC-seq footprint showing motifs enriched in primed chromatin regions from cells in the soft and stiff matrices. c mRNA expression levels of JUN, ATF3, FOS, JUNB, FOSL1, and FOSL2 in LX-2 cells cultured on soft and stiff matrix for two days (n.s. non-significant, two-tailed Wilcoxon test; two independent experiments). d Western blots and statistical results showing the protein expression levels of p-Jun in LX-2 cells cultured for two days on the soft and stiff matrices. GAPDH served as the loading control. Western-blot are representative of three independent experiments. The statistical results are shown as mean ± standard deviation (SD), one-way ANOVA. e CUT&Tag signal showing the binding of p-JUN in the different chromatin clusters defined in Fig. 2c. f Stacked bar plots showing the binding faction of p-JUN in defined chromatin region clusters. Primed chromatin is highlighted in yellow.

Back to article page