Table 1 Resources
Reagent or resource | Source | Identifier |
|---|---|---|
Antibodies | ||
 Rabbit anti-SMA | Abways | CY5295 |
 Rabbit anti-P-FOS | Cell signaling technology | 5348T |
 Rabbit anti-P-JUN | Cell signaling technology | 3270T |
 Rabbit anti-ATF3 | Abcam | ab254268 |
 Mouse anti-c-JUN | Santa Cruz Biotechnology | sc-74543 |
 Mouse anti-c-FOS | Santa Cruz Biotechnology | sc-166940 |
 Mouse anti-GAPDH | Abcam | Ab8245 |
 Rabbit anti-H3K27ac | Abcam | ab4729 |
 Rabbit anti-H3K4me3 | Abcam | ab8580 |
 Mouse anti-H3K27me3 | Abcam | ab6002 |
Biological samples | Â | Â |
 Goat anti-rabbit IgG HRP | EASYBIO | BE0101 |
 Goat anti-Mouse IgG HRP | EASYBIO | BE0102 |
 Alexa Fluor 594 donkey anti-mouse | Thermo Fisher Scientific | A21208 |
 Alexa Fluor 594 donkey anti-rabbit | Thermo Fisher Scientific | A21207 |
 Alexa Fluor 488 donkey anti-rabbit | Thermo Fisher Scientific | A21206 |
 Alexa Fluor 647 goat anti-mouse | Thermo Fisher Scientific | A21235 |
 Alexa Fluor 488 donkey anti-mouse | Thermo Fisher Scientific | A21202 |
Chemicals, peptides, and recombinant proteins | ||
 TruePrep DNA library prep kit V2 | Vazyme | TD502 |
 HiScript® II 1st strand cDNA synthesis Kit | Vazyme | R211-01 |
 NovoNGS® CUT&Tag 3.0 high-sensitivity kit | NovoProtein | N259-YH01 |
 Nuclear and cytoplasmic protein extraction kit | YEASEN | 20126ES50 |
 MolPure® cell RNA kit | YEASEN | 19231ES50 |
 ERK1/2 inhibitor | MedChemExpress | PD98059 |
 JNK1/2/3 inhibitor | MedChemExpress | SP600125 |
 P38 inhibitor | MedChemExpress | SB203580 |
Deposited data | ||
 RNA-seq, ATAC-seq, CUT&Tag | This paper |  |
 ChIP-seq | ENCODE | ENCFF781SNK, ENCFF050OMW, ENCFF760ABV, ENCFF064AZN, ENCFF055ACA, ENCFF539DCQ, ENCFF414RVB |
Experimental models: Cell lines | ||
 Human LX-2 cells | Procell | CL-0560 |
 Oligonucleotides | ||
 The primers used for qPCR can be found in the supplementary information. | This paper |  |
Software and algorithms | ||
 Bowtie2 | Langmead and Salzberg109 | |
 MACS2 | Zhang et al.110 | |
 Homer | Heinz et al.52 | |
 STAR | Dobin et al.113 | |
 DESeq2 | Love et al.104 | http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html |
 DiffBind | Stark and Brown (2011) | https://bioconductor.org/packages/release/bioc/html/DiffBind.html |
 Samtools | Danecek et al.114 | |
 Trimgalore | N/A | http://www.bioinformatics.babraham.ac.uk/projects/trim_galore |
 R | N/A | |
 ImageJ | N/A | |
 IGV | Robinson et al. (2011) | |
 chromHMM | Ernst and Kellis,42 | |
 PrimerBank | Wang and Seed115 | |
