Table 1 Enzyme activities of the TCA cycle and WLP measured in cell-free extracts in oxidative and reductive direction by spectrophotometric assays and liquid chromatography-MS (LC-MS)

From: Chemoorganoautotrophic lifestyle of the anaerobic enrichment culture N47 growing on naphthalene

Enzyme

TCA cycle

WLP

forward

reverse

Citrate synthase

2.0 ± 0.7

0.05 ± 0.008

 

Aconitase

3.6 ± 0.42*

  

Isocitrate dehydrogenase

65 ± 9.5

 

2-oxoglutarate:ferredoxin oxidoreductase

1.1 ± 0.20

  

Succinate dehydrogenase

70 ± 15

9.4 ± 0.58

 

Fumarase

0.15 ± 0.01

1.1 ± 0.46

 

Malate dehydrogenase

0.88 ± 0.24

2.9 ± 0.51

 

Carbon monoxide dehydrogenase

  

94 ± 14

Formate dehydrogenase

  

440 ± 83

  1. All activities are given in nmol min−1 mg−1 protein, errors depict measurement uncertainties of three replicate enzyme assays performed from one batch of cell free extract, taking into account the error of the protein concentration measurements that were also carried out in triplicates. Values marked with an asterisk (*) represent activities that could only be measured in combined enzyme assay reactions.
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