Fig. 3: DNAJC5 interacts with EGFR.

A Silver staining was conducted to exhibit DNAJC5 interacted proteins for Mass spectrum analysis. B The results of the LC-MS/MS analysis for the identification of potential specific DNAJC5-binding proteins were presented in the Venn diagram. C The list of the potential unique proteins with high scores that interacted with DNAJC5 via LC-MS/MS analysis. D, E The indicated plasmids expressed exogenous EGFR and DNAJC5 were co-transfected into HEK293T cells, and cell lysates were immunoprecipitated and subjected to western blot. F, G Lysates from A549 cells and PC9 cells were pulled down with anti-DNAJC5 followed by western blot. H The domains of EGFR responsible for the DNAJC5 interaction with EGFR were mapped. Schematic illustration of the domains of EGFR (up). Lysates from HEK293T cells co-transfected with the indicated plasmids were incubated with an anti-Flag antibody. The immunoprecipitate (IP) and lysates were probed with the indicated antibodies (down). I The domains of DNAJC5 binding EGFR were mapped. Schematic illustration of the domains of DNAJC5 (up). Lysates from HEK293T cells co-transfected with the indicated plasmids were immunoprecipitated and subjected to western blot (down). J The indicated plasmids expressed exogenous EGFR mutants and DNAJC5 were co-transfected into HEK293T cells, and cell lysates were immunoprecipitated and subjected to western blot.