Fig. 4: DNAJC5 enhances the activity of EGFR and downstream signaling pathways.

A A549 cells overexpressing DNAJC5 and control cells were subjected to overnight serum starvation and then treated with varying concentrations of EGF for 15 minutes. The protein levels of EGFR and phosphorylated EGFR (Tyr1068) were analyzed using western blotting. B A549 cells overexpressing DNAJC5 and control cells were subjected to overnight serum starvation followed by treatment with EGF (10 ng/ml) for different durations. C H1299 cells with knockdown of DNAJC5 expression and control cells were subjected to overnight serum starvation, followed by treatment with EGF (10 ng/ml) for 15 minutes. The protein levels of EGFR and phosphorylated EGFR (Tyr1068) were analyzed using western blotting. D H1299 cells with knockdown of DNAJC5 expression and control cells were subjected to overnight serum starvation, followed by treatment with EGF (10 ng/ml) for different durations. E, F DNAJC5 strengthens AKT and ERK signaling pathways. Cells overexpressing DNAJC5 (E), or those in which DNAJC5 was interfered with (F), underwent overnight serum starvation before being treated with EGF (10 ng/ml) for different durations. The protein levels of AKT, phosphorylated AKT, ERK, and phosphorylated ERK were analyzed using western blotting.