Fig. 5: Brown and white-grained tef accessions display differential metabolite accumulation.

a Partial least squares discriminant analysis (PLS-DA) of metabolites in grain samples of brown and white-grained accessions. Ellipses represent 95% confidence intervals around each group. b Differentially accumulated metabolites show enrichment for several metabolic pathways, notably fatty acid and flavone metabolism. Point size scales with pathway impact and colour intensity scales with significance of pathway enrichment. c Volcano plot for the 183 identifiable differentially accumulated metabolites. Fold-change (FC) was calculated as mean value in brown-grained varieties divided by that in white-grained varieties. Plotted FC thresholds are log2(0.83) and log2(1.2) and plotted FDR threshold is log10(0.05).