Fig. 2: JMY is required for proper dendritic arborization.

A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of 3D morphometric reconstructions by using Imaris software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points (B), dendritic terminal points (C), total dendritic tree length (D), as well as by conducting dendritic branch depth (E) and Sholl analyses (F). Data, mean ± SEM shown as bar/dot plots (B–D) and bar plot (E, F), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest (B–D) and two-way ANOVA/Bonferroni′s test (E, F), respectively. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. For exact p values see figure panels. Note that ****p < 0.0001 are too small values to be reported by the software used.