Fig. 3: Development of the dendritic arbor depends on both the N- and C-terminus of JMY. | Communications Biology

Fig. 3: Development of the dendritic arbor depends on both the N- and C-terminus of JMY.

From: JMY powers dendritogenesis and is regulated by CaM revealing a general, critical principle in neuromorphogenesis

Fig. 3

A Schematic representation of JMY and mutant variants thereof used to rescue JMY loss-of-function phenotypes. B MIPs of anti-MAP2-immunostained primary hippocampal neurons transfected at DIV4 with either scr. RNAi, RNAi#1, RNAi#1/JMY*, RNAi#1/JMY* ΔCT, and RNAi#1/JMY* ΔNT, respectively, and fixed 30 h after transfection. Transfected cells are expressing GFP as reporter (see Supplementary Fig. 3A) and are marked by asterisks. Quantitative analyses of dendritic branch points (C), dendritic terminal points (D), and total dendritic length (E). In comparison to neurons transfected with RNAi#1, only coexpression of wild-type JMY* but not JMY* ΔCT or JMY* ΔNT rescued the JMY loss-of-function phenotypes shown by dendritic branch point, dendritic terminal point, and dendritic length analyses. F MIPs of hippocampal neurons transfected and fixed under the same conditions as for the morphometric analyses (B–E) with additional anti-JMY immunostainings and DAPI stainings showing that the mutants not able to rescue were successfully expressed and available in soma and dendrites. Bars, 30 µm. Data, mean ± SEM shown as bar/dot plots. Scr. RNAi, RNAi#1 and RNAi#1/JMY* n = 75 neurons (partially included in Fig. 2, too); RNAi#1/JMY* ΔNT and RNAi#1/JMY* ΔCT, n = 45 neurons each from 2 to 5 independent assays. Numerical data are provided in Supplementary Data 1. Significant differences, One-way ANOVA/Tukey’s post-test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. For exact p values see figure panels. Note that ****p < 0.0001 are too small values to be reported by the software used.

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