Fig. 7: The functions of JMY in dendritic arborization require proper Ca2+/CaM signaling.
A Representative MIPs of the GFP and anti-MAP2 signals of primary hippocampal neurons transfected with either GFP or JMY*/GFP at DIV4 and treated with the CaM inhibitor CGS9343B and solvent control (DMSO), respectively. Asterisks mark transfected cells. Bars 30 µm. B–E Imaris software-based reconstruction and quantitative analyses of the dendritic arbors of transfected neurons 30 h after transfections. Shown are determinations of dendritic branch points (B), dendritic terminal points (C), and total dendritic length (D) as well as Sholl analyses of dendritic arbor complexity (E). Note that all JMY gain-of-function phenotypes were completely abolished upon the addition of CGS9343B when compared to solvent control. Data, mean ± SEM shown as bar/dot plots (B–D) and bar plots (E), respectively. GFP+DMSO and JMY*/GFP+DMSO (same data as in Fig. 4B–E), n = 40 each; GFP+CGS9343B and JMY*/GFP+CGS9343B n = 30 cells each from 2 independent assays and neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, Two-way ANOVA, Sidak's test (B-D) and Three-way ANOVA with Tukey’s multiple comparisons test (E), respectively, for the factor transfection (GFP vs. JMY*/GFP shown with *) and the factor treatment (DMSO vs. CGS9343B shown with #). *p < 0.05, ***p < 0.001, ****p < 0.0001, #p < 0.05, ####p < 0.0001. For exact p values in B-D see figure panels. Note that ****p < 0.0001 are too small values to be reported by the software used.
