Fig. 9: Regulation of post-mating behaviors of females by neuropeptide signaling pathways in males.

a, b Effects of AT and sNPF knockdown (a), or ATR and sNPFR knockdown (b) in males on the number of eggs laid by the females during 24 h after mating with males. c, d Effects of AT and sNPF knockdown males (c), or ATR and sNPFR knockdown (d) on the hatchability of the eggs laid by the females. Values are shown as mean ± SEM, n = 5–27, Sidak’s test, *P < 0.05, ***P < 0.001. ns not significant. SV, seminal vesicle. e, f Effects of AT and sNPF knockdown (e), or ATR and sNPFR knockdown (f) in males on the duration of remating of the mated females. Values are shown as mean ± SEM, n = 13–18, Dunnett’s test, *P < 0.05. ns not significant.