Fig. 6: Transcriptomics of RPE/choroid reveals partial recovery of declined tight junctions and attenuation of upregulated inflammatory signals in 8-AG-treated rats.
From: Oral 8-aminoguanine against age-related retinal degeneration
RPE/choroids of young, aged, and 8-AG-treated aged rats were manually separated from the retinae were processed for transcriptomic analysis using bulk RNA-seq. Aged untreated vs. young and aged 8-AG treated vs. aged untreated DEGs were analyzed by GO to identify affected biological pathways (BPs). DEGs were identified with >1.5-fold changes with P values < 0.05. N = 3. a, b are up- and downregulated BPs, respectively, in RPEs/choroids from aged untreated vs. young rats. c, d are up and downregulated BPs, respectively, in RPEs/choroids in aged 8-AG-treated vs. aged untreated rats. The X axis represents the DEGs percentage in a gene set, and the Y axis represents the BPs. Right side of the graphs, shows the corresponding cellular pathways.
