Fig. 5: HG CRC cell lines lose expression of markers of LGR5+ colon stem cells and gain expression of markers of fetal-like intestinal stem cells. | Communications Biology

Fig. 5: HG CRC cell lines lose expression of markers of LGR5+ colon stem cells and gain expression of markers of fetal-like intestinal stem cells.

From: Model systems and unique biological features of high and low-grade colorectal cancer (CRC) revealed by xenografting 84 human CRC cell lines

Fig. 5

A Downregulation of LGR5+ colon stem cell signature in HG CRC cell lines. B Expression (mRNA) of LGR5+ colonic stem cell markers in a subset of HG and LG CRC cell lines determined by qPCR. Values shown are mean + SEM of a representative experiment performed in technical triplicate. C Expression of corresponding LGR5+ colonic stem cell markers in HG (n = 58) vs LG (n = 202) primary CRCs in the TCGA cohort (RNAseq data). D Enrichment of fetal-like intestinal stem cell signature33 in HG CRC cell lines. E mRNA expression of fetal-like stem cell markers in HG and LG CRC cell lines determined by qPCR. Values shown are mean + SEM of a representative experiment performed in technical triplicate. F Corresponding mRNA expression of fetal-like intestinal stem cell markers in HG (n = 58) vs LG (n = 202) primary CRCs in the TCGA cohort (RNAseq data). Values shown in (C and F) are mean + SEM, and groups were compared using unpaired t-tests. **P < 0.01, ***P < 0.005, ****P < 0.001.

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