Fig. 4: Absence of UBE3A disrupts progenitor proliferation and rosette structure.
From: Loss of UBE3A impacts both neuronal and non-neuronal cells in human cerebral organoids
A Flow cytometry plots of EdU labeled H9WT, H9UBE3A m−/p+ and H9UBE3A m−/p− embryoid bodies and B fraction of cells in different cell cycle states based on EdU assay performed. ***p-value < 0.001 and ****p-value < 0.0001 via one-way ANOVA with Tukey-Kramer post hoc analysis, (ns not significant, p-value > 0.05), n = 3 replicates of 15 pooled embryoid bodies per genotype. Full tick mark indicates sample being compared to half tick mark samples. Error bars are 95% confidence intervals. C Flow cytometry plots of EdU labeled H9WT, H9UBE3A m−/p+ and H9UBE3A m−/p− pluripotent stem cells and D fraction of cells in different cell cycle states based on EdU assay. ***p-value < 0.001 via one-way ANOVA with Tukey-Kramer post hoc analysis, (ns not significant, p-value > 0.05), n = 3 replicates of pluripotent stem cells from independent culture dishes. Full tick mark indicates sample being compared to half tick mark samples. Error bars are 95% confidence intervals. E Brightfield images of H9WT and H9UBE3A m−/p− organoid size comparison. Scale bars = 200 μm. F Size comparison quantification between 3 week old H9WT and H9UBE3A m−/p− organoids. **p-value < 0.01 via one-tailed t-test, n = 3 independent batches, each with ≥11 organoids per genotype. Error bars are 95% confidence intervals. G EMX1 expression levels in H9WT and H9UBE3A m−/p− organoids based on 6 week scRNAseq data. H Immunostaining of H9WT, H9UBE3A m−/p+ and H9UBE3A m−/p− human cortical organoids with EMX1, MAP2 and SOX2. Scale bars = 250 μm. I Immunostaining of H9WT, H9UBE3A m−/p+ and H9UBE3A m−/p− human cortical organoids with EMX1, MAP2 and GLI3. Scale bars = 250 μm.
