Fig. 5: Lack of UBE3A may disrupt cortical layer-like EN development.
From: Loss of UBE3A impacts both neuronal and non-neuronal cells in human cerebral organoids
A UMAP bidimensional reduction of 11 week organoid scRNAseq with cell type identities. RG radial glia, IN inhibitory-like neuron, EN excitatory-like neuron, ChP choroid plexus, IP intermediate progenitor, EN-CL cortical layer-like neurons, Mes mesenchymal, HSP heat shock protein. B UMAP of 11 week H9WT and H9UBE3A m−/p− organoids. C Cell type proportions of 11 week H9WT and H9UBE3A m−/p− organoids. *q-value < 0.05, **q-value < 0.01, ***q-value < 0.001 and ****q-value < 0.0001, via t-test using the arcsin square root variance stabilizing transformation propeller test (Speckle116 v1.2.0), n ≥ 3 replicates of 4–5 pooled organoids per genotype. D UMAP highlighting cell types with altered proportions between H9WT and H9UBE3A m−/p– organoids. E Organoid composition bar plots for highlighted cell types. *q-value < 0.05, **q-value < 0.01, ***q-value < 0.001 and ****q-value < 0.0001, via t-test using the arcsin square root variance stabilizing transformation propeller test (Speckle v1.2.0), n ≥ 3 replicates of 4–5 pooled organoids per genotype. Error bars are 95% confidence interval.
