Fig. 6: Cell type specific transcriptomic differences between 11 week H9WT and H9UBE3A m−/p− organoids. | Communications Biology

Fig. 6: Cell type specific transcriptomic differences between 11 week H9WT and H9UBE3A m−/p− organoids.

From: Loss of UBE3A impacts both neuronal and non-neuronal cells in human cerebral organoids

Fig. 6

A Volcano plot of bulk DEGs between H9UBE3A m−/p− and H9WT organoids. B Number of up and downregulated bulk DEGs between H9UBE3A m−/p– and H9WT organoids. DEG defined as log2FC > |1|, and false discovery rate q < 0.05. C Number of up and downregulated DEGs separated by cell type. DEG defined as log2FC > |1|, and false discovery rate q < 0.05. RG radial glia, IN inhibitory-like neuron, EN excitatory-like neuron, ChP choroid plexus, IP intermediate progenitor, Mes mesenchymal, HSP heat shock protein. D Top 50 canonical pathways identified through QIAGEN Ingenuity Pathway Analysis (IPA) grouped by category. Differentially expressed genes with false discovery rate q  <  0.05, log2FC  ≥ |1| were used in this analysis. Pathways related to cancer or inherently from outside the nervous system were omitted. Please refer to Supplementary Data 5 for all pathways.

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