Fig. 7: Excess ChP generation in the absence of UBE3A.
From: Loss of UBE3A impacts both neuronal and non-neuronal cells in human cerebral organoids
A Brightfield images showing fluid-filled cyst and sinuous tissue morphologies in organoids. Scale bars = 200 μm. B Quantification of ChP-like morphologies for 3 week old whole brain organoids. n = 3 independent organoid batches each with ≥11 organoids per genotype. CHP choroid plexus. Full tick mark indicates sample being compared to half tick mark samples. **p-value < 0.01 via one-way ANOVA with Tukey-Kramer post hoc analysis, (ns not significant, p-value > 0.05). Error bars are 95% confidence intervals. C TTR immunostaining in fluid-filled cysts and invaginations. Scale bars = 500 μm. D Canonical pathways deregulated by the absence of UBE3A in 6 week and 11 week organoid scRNAseq data. Top 25 canonical pathways are shown. Differentially expressed genes with false discovery rate q  <  0.05, log2FC  ≥ |1| were used in this analysis. Pathways related to cancer or inherently from outside the nervous system were omitted. Please refer to Supplementary Data 2 and 5 for all pathways. E Spatiotemporal localization of UBE3A in ChP-like tissue. Distinct nuclear UBE3A observed in early H9WT organoids weakens over time in TTR+ ChP cells. Scale bars = 25 μm.
