Fig. 1: Eight weeks of high-fat diet disrupted glucose metabolism for young and aged mice of both sexes but only increased mass for males.

a Percent mass change from the start to end of the diet intervention and b the reproductive adipose depot mass at harvest. c The area under the intraperitoneal glucose tolerance testing (ipGTT) curves was termed as the blood glucose area under the curve (AUC), d the first time point (0 min.) following 6 h of fasting was the fasting blood glucose level. The mean ipGTT curves ± 1 SD for each diet group of the e young females, f the aged females, g the young males, and h the aged males. Line plots indicate the mean value for a group ± 1 SD with faded points indicating individual data points (triangles are low-fat diet (LFD) points and circles are high-fat diet points (HFD). Boxplots illustrate the interquartile range (box), the minimum and maximum values (whiskers), the median value (bar across the box), the mean value (white square), and individual data points (black circles). Colors indicate groups where light gray is young LFD females, dark gray is young HFD females, light blue is aged LFD females, dark blue is aged HFD females, light yellow is young LFD males, dark yellow is young HFD males, light red is aged LFD males, and dark red is aged HFD males. Significant p-values from 3-way ANOVA and pairwise post-hoc tests are noted on the plots. For all models unrelated to ipGTT, the glucose AUC was considered as a covariate in a 3-way ANCOVA model. If the covariate was not significant, the model was rerun without it. N = 7–11 animals/group without outliers, but statistical results with specific n per group for each measure can be found in Supplementary Data 1.