Fig. 7: Bone matrix composition depends on high-fat diet in an age- and sex-dependent manner.

The measurements of matrix maturity from hydrated Raman spectroscopy for the periosteal surface and the endocortical region (anterior-lateral location). Column 1—the median measures for the periosteal surface (P. Median). Column 2—the median measures for the endocortical region (E. Median). Column 3—the gradient (slope of linear fit) of the measures across the endocortical region (E. Gradient). Column 4—estimate of the root mean square deviation (RMSD) from linear fit residuals of each measure across the endocortical region (E. RMSD). Boxplots illustrate the interquartile range (box), the minimum and maximum values (whiskers), the median value (bar across the box), the mean value (white square), and individual data points (black circles). Colors indicate groups where light gray is young LFD females, dark gray is young HFD females, light blue is aged LFD females, dark blue is aged HFD females, light yellow is young LFD males, dark yellow is young HFD males, light red is aged LFD males, and dark red is aged HFD males. Significant p-values from 3-way ANOVA and pairwise post-hoc tests are noted on the plots. For all models, the glucose AUC was considered as a covariate. If not significant, the model was rerun without the covariate. N = 3–4 animals/group for females and N = 7–10 animals/group for males without outliers, but statistical results with specific n per group for each measure can be found in Supplementary Data 1.