Fig. 3: Spatial transcriptomics identify spatially restricted genes during the transition from mitosis to meiosis and follicle formation. | Communications Biology

Fig. 3: Spatial transcriptomics identify spatially restricted genes during the transition from mitosis to meiosis and follicle formation.

From: Spatiotemporal and single-cell atlases to dissect regional specific cell types of the developing ovary

Fig. 3

a UMAP clustering analysis of ovarian germ cells at E13.5 (left) and marker genes (right). b Volcano plot showing the significantly differentially expressed transcripts in the meiotic FGCs compared with the mitotic FGCs. c GO terms of differentially expressed transcripts between meiotic FGCs and mitotic FGCs at E13.5. d Visualization of mitotic FGCs and meiotic FGCs on E13.5 mouse ovary based on integration of scRNA-seq cell type annotations with spatial transcriptomic data (left); Spatially resolved gene expression for region restricted genes (right). A: Anterior region; P: Posterior region. e Immunostaining for the POU5F1 and SYCP3 in ovaries at E13.5. The scale bars represent 100 μm. A: Anterior region; P: Posterior region. f Spatially resolved gene expression for region restricted genes at E13.5 ovary. g Spatially resolved genes enriched in cortical and medullary regions (left). GO terms of differentially expressed genes at MSR and CSR (right). SR spatial region, MSR medullary spatial region, CSR cortical spatial region, SR&SC spatial region and single cell transcriptomes.

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