Fig. 2: circGIGYF1 regulated CRC cell EMT, stemness, and metastasis. | Communications Biology

Fig. 2: circGIGYF1 regulated CRC cell EMT, stemness, and metastasis.

From: circGIGYF1 inhibits stemness and metastasis in colorectal cancer by promoting WWP2-HOXD13 interaction to regulate β-catenin signalling

Fig. 2

A qRT-PCR analysis of circGIGYF1 and linear GIGYF1 mRNA expression in LoVo and HCT116 cells overexpressed circGIGYF1 and SW620 cells silenced circGIGYF1. B Sphere formation of cells overexpressed or silenced circGIGYF1 was detected using sphere formation assay. Scale bar: 100 μm. C qRT-PCR analysis of stemness genes (OCT4, Nanog, and Sox2) in cells overexpressed or silenced circGIGYF1. D The percentage of CD133+ cells was analyzed in cells transfected with circGIGYF1 or shcircGIGYF1 by flow cytometry. E, F Tumor cell migratory and invasive capacities were detected by wound-healing and Transwell assays after overexpressing or silencing circGIGYF1. Scale bar: 500 μm or 100 μm. G The expression of EMT-related markers (N-cadherin, E-cadherin, and Vimentin) in CRC cells was measured by Western blotting after overexpressing or silencing circGIGYF1. GAPDH was used as an internal control. Mean ± SD, n = 3, **p  <  0.01, ***p  <  0.001. Statistical analysis was carried out by a one-way ANOVA.

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