Fig. 2: Frequency response characteristics of Kcnq2/3 currents.
From: Kcnq (Kv7) channels exhibit frequency-dependent responses via partial inductor-like gating dynamics
a Waveform of the sinusoidal input voltage. b–e Representative currents recorded in control (upper traces, green) and XE991-containing (20 μM, upper traces, orange) solutions in response to the sinusoidal input voltage (± 15 mV) at −40 mV in Kcnq2/3-expressing HEK293 cells. XE991-sensitive currents (lower traces, blue) were calculated by subtracting the current obtained in the XE991-containing solution from that in the control solution. These recordings were performed with input voltage frequencies of 0.5 (b), 5 (c), 10 (d), and 15 (e) Hz. f Amplitudes of currents recorded in control (n = 7, green) and XE991-containing (n = 7, orange) solutions plotted against the holding potential frequency. The half-magnitude from the bottom to the top of the sinusoidal membrane current represents the amplitude. Small dots represent individual data and large dots with bars represent the mean ± SEM. ***P < 0.001 (repeated measures two-way ANOVA; post-hoc Holm–Šidák test). g, h Frequency dependence of outward (g) and inward (h) current amplitudes of XE991-sensitive currents (n = 7). Small light-blue dots represent individual data, and large blue dots with bars represent the mean ± SEM. **P < 0.01, *P < 0.05 (repeated measures one-way ANOVA; post-hoc Holm–Šidák test).
