Fig. 4: Deconvolution analysis and pathway enrichment analysis of the periosteum region across fracture healing stages. | Communications Biology

Fig. 4: Deconvolution analysis and pathway enrichment analysis of the periosteum region across fracture healing stages.

From: Integrating spatial and single-cell transcriptomics to characterize mouse long bone fracture healing process

Fig. 4

A Sequential histological sections from Day 0, Day 5, and Day 15 postfracture showing the periosteum region. Below each histological image, spatial plots and corresponding deconvolution results using the CARD package are presented, illustrating the inferred cellular composition within each spatial spot. B Heatmaps displaying the results of GSVA for different cell clusters identified in the periosteum across Days 0, 5, and 15. Each row represents a KEGG or Hallmark pathway. C SCENIC analysis results showing the activity of transcriptional regulators across different periosteal cell types and time points. This analysis reveals the regulatory landscapes that potentially guide the differentiation and function of cells in response to fracture.

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