Fig. 3: Deletion of Pcm1 impairs spermatogenesis and spermiogenesis.
From: PCM1 orchestrates centrosomal and flagellar protein transport to promote sperm maturation
a Immunostaining of DDX4 (green) to label germ cells in Pcm1+/+ and Pcm1−/− testes. Scale bars: 50 μm. b Immunostaining of γH2AX (green) showed germ cell injuries in Pcm1+/+ and Pcm1−/− testes. Scale bars: 50 μm. c TUNEL (green) assay showed germ cell apoptosis in Pcm1+/+ and Pcm1−/− testes. Scale bars: 10 μm. d Quantification of DDX4 fluorescence in (a), n = 6 for each group. e Quantification of γH2AX fluorescence in (b), n = 6 for each group. f Quantification of TUNEL-positive cells in seminiferous tubules. n = 8. g Immunofluorescence of acetylated tubulin (Ac-Tub, green) to label spermatid flagella in the seminiferous tubules of Pcm1+/+ and Pcm1−/− mice. Scale bars: 50 μm (up), 20 μm (down). h PAS staining of testes sections showed enlarged lumen and absence of flagella in the Pcm1−/− tubules. Scale bars: 20 μm. i PAS staining of spermatids at different steps from the Pcm1+/+ and Pcm1−/− mice. Notable morphological abnormalities in the Pcm1−/− spermatids were first seen at step 9. Scale bars: 5 μm. Blue indicate nuclei stained with DAPI. Mice used were 10 weeks old. Data were presented as Mean ± SD. t-test was used for comparing two groups.
