Fig. 6: Role of TRN in modulating sound-evoked responses in the MD. | Communications Biology

Fig. 6: Role of TRN in modulating sound-evoked responses in the MD.

From: The neural circuit mechanism for auditory responses in the mediodorsal thalamic nucleus of awake mice

Fig. 6

A Schematic for optogenetic inactivation of TRN (TRN-silencing), and simultaneous SUA and LFP recordings in MD of GAD2-Cre mice that received AAV-DIO-eNpHR3.0-mCherry injection in the TRN. Created by the authors. B Raster plots and PSTHs of three representative MD neurons with and without TRN-silencing. Quantification of spontaneous firing rates (C) and AUC of sound-evoked Phasic- (D) and Sustained-responses (E) in MD neurons under different conditions. N = 69 units from 8 mice. Representative traces of LFP (F) and corresponding time-frequency spectrograms (G) in response to sound stimuli with and without TRN-silencing. Normalized PSD of LFPs in the 0–80 ms (H) and 80–500 ms (I) post-stimulus window. Statistical analysis of normalized PSD across frequency bands in the 0–80 ms (J) and 80–500 ms window (K). N = 16 recording sessions from 8 mice. **P < 0.01, as determined by an LME.

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