Table 1 Microtubule dynamics simulation parameters

[GTP-tub]

Free GTP-tubulin concentration

8.14 µM

[GMPCPP-tub]

Free GMPCPP-tubulin concentration

0 M (assumption)

k_on,MT

Tubulin on-rate constant per microtubule

65 μM⁻¹ s⁻¹microtubule^{−126,27,54,55}

k_{hyd, GTP}

Hydrolysis rate constant per GTP-tubulin subunit

0.2–0.5 s⁻¹ subunit⁻¹

k_{hyd, GMPCPP}

Hydrolysis rate constant per GMPCPP-tubulin subunit

~0 s⁻¹ subunit⁻¹⁵⁵

ΔG^0*_Long,GTP

Longitudinal chemical bond energy (GTP-tubulin polymer)

−7.2k_BT^26,27,54,55

ΔG⁰_Lat,GTP

Lateral chemical bond energy (GTP-tubulin polymer)

−5.7k_BT^26,27,54,55

ΔG^0*_Long,GMPCPP

Longitudinal chemical bond energy (GMPCPP-tubulin polymer)

−7.2k_BT (matched to GTP)

ΔG⁰_Lat,GMPCPP

Lateral chemical bond energy (GMPCPP-tubulin polymer)

−5.7k_BT (matched to GTP)

EI_MT

Flexural rigidity of a microtubule

4 × 10⁻²⁴  N m²²⁷

θ_GTP,pref

Preferred angle for GTP-tubulin

0°²⁷

θ_GDP,pref

Preferred angle for GDP-tubulin

22°²⁷

θ_GMPCPP,pref

Preferred angle for GMPCPP-tubulin

0° (assumed)