Fig. 2: In vivo LTP induction in the PB-CeC/L pathway enhances avoidance behavior and subsequent aversive learning. | Communications Biology

Fig. 2: In vivo LTP induction in the PB-CeC/L pathway enhances avoidance behavior and subsequent aversive learning.

From: Aversive experiences induce valence plasticity of instructive signals to change future learning rules in mice

Fig. 2

a Schematic of AAV injection into the PB and cannulation into the CeC/L. b Representative image of Chronos:GFP expression in the CeC/L. The dashed line indicates the position of the cannula. The scale bar represents 500 μm. c Behavioral scheme for successive conditioning paradigms. TC threat conditioning, Ctx context, CS conditioned stimulus; U, unconditioned stimulus. eYFP control group (n = 7); Chronos group (n = 8). d Time course of the freezing ratio in each mouse in both groups in the retrieval test. Color codes show the freezing level at each time point (bin = 5 s). BL, baseline; eYFP, Control group; Chronos, Chronos group. e Average freezing ratio in the retrieval test. Baseline freezing is represented before CS presentation. f Behavioral scheme for the real time place aversion (RTPA) paradigm (eGFP, n = 8; Chronos, n = 8). g Stay time in the shock area during RTPA. h Stay time in the control area during RTPA. i Aversion index (control area/Shock area) during RTPA. e Two-way repeated-measures ANOVA and Bonferroni’s multiple comparisons test. g, h Mann–Whitney test. i Two-tailed unpaired t-test. ns, p > 0.05; *p < 0.05; ****p < 0.0001. Data are presented as mean ± S.E.M.

Back to article page