Fig. 1: Changes in individual genes with conditional MR KO are evident in spatial transcriptomics and single neurons.
From: Mineralocorticoid receptor knockout alters hippocampal CA2 neurons to become like those in CA1
a Examples of brains from a Cre- WT and a Cre+ MR KO mice showing expression of individual genes displayed using the Loupe Browser (10x Genomics). Shown are ‘CA2 genes’ Amigo2, Necab2, and Pcp4, as well as a ‘low in CA2’ gene Prkcb. Also shown are the ‘CA1 gene’ and ‘CA3 gene’ Wfs1 and Iyd respectively. Data are not normalized to spot read counts in these displays. Orange/red = higher expression. b Images showing gene expression using single molecule fluorescence in situ hybridization (smFiSH) to validate changes in gene expression with MR KO. Shown are Necab2, Pcp4, and Prkcb. Note the loss of Necab2 and Pcp4, and the filling in with Prkcb in the area presumed to be CA2 of the MR KO. c smFISH showing that single neurons express neither Wfs1 nor Iyd in CA2 of WT mice. However, neurons in the presumed CA2 region in the MR KO express the CA1 gene Wfs1, but not the CA3 gene Iyd, with rare examples of neurons expressing both.
