Fig. 3: In vitro activity of the reference drugs against L. donovani DD8 intracellular amastigotes.

A Amphotericin B, B miltefosine and C VL-2098. The percentage inhibition was determined from quadruplicate assay data points after normalization of the data with 1 µM amphotericin B (100% death, final assay) as the positive control and 0.4% DMSO (0% inhibition) as the negative control. Left-Y-axis: percentage inhibition of L. donovani DD8 (blue dots/triangles and curves); Right-Y-axis: percentage inhibition of THP-1 cells (red dots/triangles and curves); X-axis: Log of micromolar compounds concentration. Data points are means and error bars represent the standard deviations of two independent experiments (dots for experiment 1 and triangles for experiment 2). Individual data points are represented as lighter-shaded markers in the corresponding colour of each group.