Fig. 5: Microglia response in perilesioned area.
From: A novel model of light-induced middle cerebral artery occlusion and recanalization in mice
a Representative confocal images for the microglia (Iba1 in red) and phagocytosis activity (CD68 in white). Scale bar 50 µm. b The graph shows the microglia density (mean ± SEM) of Iba1-positive cells in the three experimental groups (Sham n = 3; PT n = 6; PTRC n = 6; Sham=107.3 ± 13.41; PT = 281.5 ± 14.69; PTRC = 167.6 ± 19.20). *p value based on one-way ANOVA followed by Tukey’s post hoc test for the intragroup statistical analysis (F(2, 12) = 23.22 p < 0.0001; Sham vs PT p = 0.0001; PT vs PTRC p = 0.0008). c The graph shows the CD68 area (mean ± SEM) in the three experimental groups (Sham n = 3; PT n = 6; PTRC n = 6; Sham = 3.73 ± 0.62; PT = 42.72 ± 8.29; PTRC = 8.84 ± 2.13). *p value based on one-way ANOVA followed by Tukey’s post hoc test for the intragroup statistical analysis (F(2, 12) = 12.57 p = 0.0011; Sham vs PT p = 0.0041; PT vs PTRC p = 0.0026). d The graph shows the Iba1 area (mean ± SEM) in the three experimental groups (Sham n = 3; PT n = 6; PTRC n = 6; Sham = 49.38 ± 9.36; PT = 726.8 ± 71.12; PTRC = 337.0 ± 32.78). *p value based on one-way ANOVA followed by Tukey’s post hoc test for the intragroup statistical analysis (F(2, 12) = 33.00 p < 0.0001; Sham vs PT p < 0.0001; Sham vs PTRC p = 0.017; PT vs PTRC p = 0.0004).
