Fig. 6: Timely recanalization of occluded MCA reduces the insurgence of cerebral edema.
From: A novel model of light-induced middle cerebral artery occlusion and recanalization in mice
a Schematic representation of the experimental procedure to evaluate brain water content. b Experimental timeline for the second set of experiments in Sham (n = 5), PT (n = 4), and PTRC (n = 5) mice. c The graph shows a comparison between Sham, PT and PTRC groups of brain water content in the left (injured/operated) and right hemispheres. Following mean and SEM values in the different experimental groups: Sham IL = 63.3 ± 0.4 Sham CL = 64.0 ± 0.9; PT IL = 68.8 ± 0.9, PT CL = 65.1 ± 0.6; PTRC IL = 65.9 ± 1.0, PTRC CL = 67.2 ± 1.8. *p value based on Two-way ANOVA (F(5, 22) = 4.206) followed by post hoc Tukey HSD test: PT IL-CL p = 0.147; Sham IL-PT IL p = 0.035. d The graph shows variation in mice weight between pre-stroke conditions and basal time point, and between basal conditions and 24 h after surgery. Following mean and SEM values in the different experimental groups: Sham: basal = 0.4 ± 0.2; 24 h = 1.4 ± 0.5; PT: basal = 0.8 ± 0.4; 24 h = 2.6 ± 0.7; PTRC: basal = 0.1 ± 0.6; 24 h = −1.1 ± 0.3. e The graph highlights an increase in neurological deficit scores in the Sham, PT, and PTRC groups 24 h after surgery. Following mean and SEM values in the different experimental groups: Pre: Sham = 0, PT = 0, PTRC = 0; 24 h: Sham = 0; PT = 11 ± 1.1; PTRC = 5.6 ± 1.7. *p value based on Two way ANOVA repeated measure followed by post hoc Tukey HSD test (F(5, 22) = 50.991): PT Pre-24h p < 0.00001; PTRC Pre-24h p = 0.004; Sham 24h-PT 24 h p < 0.00001; Sham 24h-PTRC 24 h p = 0.006; PT 24h-PTRC 24 h p = 0.008. f Correlation fit of neuro deficit score and brain water content Pearson correlation r = 0.640; p value = 0.013.
