Fig. 6: Alterations of Q/R/N and + 4 sites differentially regulate internal polyamine block in T. adhaerens GluE1αA receptors. | Communications Biology

Fig. 6: Alterations of Q/R/N and + 4 sites differentially regulate internal polyamine block in T. adhaerens GluE1αA receptors.

From: Evolution of iGluR ligand specificity, polyamine regulation, and ion selectivity inferred from a placozoan epsilon receptor

Fig. 6

a, d, g, j Raw current traces evoked by 250 ms exposure to 10 mM glycine with co-concurrent voltage steps (range, −100 to 100 mV; Δ10 mV). Sample traces correspond to the following: GluE1αA (a), GluE1αA S642Q (d), GluE1αA S642N (g), and GluE1αA D646A (j). b, e, h, k Mean I-V plots for each receptor complex ( ± standard deviation), normalized to maximum current response at −100 mV. Data points for single replicates are included in the plot (GluE1αA, n = 5; GluE1αA S642Q, n = 10; GluE1αA S642N, n = 8; GluE1αA D646A, n = 7). c, f, i, l Mean G-V plots for each receptor complex ( ± standard deviation), normalized to their respective maximal conductance. Cyan lines represent fitting using the single permeant blocker model, apart from GluE1αA D646A, which was fit with an exponential equation (described in Methods).

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