Table 1 Equilibrium spermine block using the single permeant blocker model

From: Evolution of iGluR ligand specificity, polyamine regulation, and ion selectivity inferred from a placozoan epsilon receptor

Receptor

Kd(0mV) (µM)

h (mV)

k (mV)

n

GluA2(Q) wt

5.4 ± 2.6

−18.5 ± 0.7

14.3 ± 0.6

6

GluA2 Q607S

36.2 ± 6.7 (***, a)

−14.0 ± 2.3

164.4 ± 31.1

4

GluA2(Q) wt + γ2

38.4 ± 11.9

−16.7 ± 1.9

18.2 ± 3.0

5

GluA2 D611A + γ2

168.7 ± 54.6(**)

−58.8 ± 8.8

20.8 ± 3.7

4

GluE1αA wt

11.4 ± 1.9

−18.5 ± 0.6

36.5 ± 4.7

5

GluE1αA S642Q

5.7 ± 3.7 (**)

−15.3 ± 0.7

28.3 ± 2.1

10

GluE1αA S642N

8.0 ± 2.9

−26.4 ± 1.3

34.9 ± 3.7

8

GluE1αA D646A

-

-

-

7

  1. Kd(0mV) refers to the dissociation constant of Spm and are listed in micromolar (μM). Voltage dependency of block onset (h) and block relief (k) are listed as millivolts (mV). The number of patch recordings for each receptor (n) is indicated. Comparisons are drawn between mutated constructs and the corresponding wildtype receptor, unless otherwise indicated. GluA2 Q607S Kd(0mV): t(8) = −10.3, ***p < 0.001, unpaired two-tailed Student’s t test. Kd(0mV) of GluA2 Q607S versus GluE1αA wt: t(7) = 7.93, ap < 0.001, unpaired two-tailed Student’s t test. GluA2 D611A Kd(0mV): t(7) = −5.23, **p = 0.00117, unpaired two-tailed Student’s t test. Kd(0mV) of GluE1αA constructs: F(2,19) = 5.23, p = 0.0129, one-way ANOVA. Tukey’s HSD test, **p = 0.00972. All values represent mean ± standard deviation.
Back to article page