Fig. 2: Calibration setup and fluorescent cell images captured with our imaging unit. | Communications Biology

Fig. 2: Calibration setup and fluorescent cell images captured with our imaging unit.

From: A modular fluorescent camera unit for wound imaging

Fig. 2

a Calibration and in vitro imaging setup. The imaging unit is placed face up, and a linear stage controlled by a dial is used to hold and position the sample. b Fluorescent imaging of THP-1 cells. The full image is shown with a 1000 μm scale bar, and the inlet is zoomed in with a 100 μm scale bar. The inlet images are color-mapped to increase the contrast of the fluorescent signal. Control: control slide with no dye. Calcein, AM: cells dyed with Calcein AM fluorescent compound. DAF-FM: cells dyed with DAF-FM fluorescent compound. c Fluorescent imaging of macrophage (M1) cells. The full image is shown with a 1000 μm scale bar, and the inlet image is zoomed in with a 100 μm scale bar. Control: control slide with no dye, DAF-FM: cells dyed with DAF-FM fluorescent compound.

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