Fig. 2: Presence of ENaC-coding SCNN1 genes in Cetartiodactyla.
Presence or pseudogenisation of SCNN1 genes was investigated in the highlighted species using the National Center for Biotechnology Information (NCBI) database. Blue squares indicate intact genes yielding continuous open reading frames (ORFs), whereas magenta squares indicate pseudogenes containing multiple insertions/deletions and splice donor/acceptor site mutations causing preliminary STOP codons. Turquoise squares highlight species containing a fusion of exons 11 and 12 due to a mutation of the splice donor following exon 11, which did not disrupt the ORF. Grey squares highlight incomplete genomic information. There is currently no available genomic information of species representing the family Neobalaenidae. Check mark symbols = Coding sequences or erosion of SCNN1D was confirmed using transcriptomic data available at the NCBI Sequence Read Archive. Accession numbers are provided in Supplementary Table 1. ° = transcriptomic data incomplete. The phylogenetic tree was created based on Cabrera et al.69. Background colour code: light blue = marine species; turquoise = amphibious species; green = terrestrial species.
