Fig. 6: Presence of ENaC-coding SCNN1 genes in Afrotheria and Carnivora.

Presence or pseudogenisation of SCNN1 genes was investigated in the highlighted species using the National Center for Biotechnology Information (NCBI) database. Blue squares indicate intact genes yielding continuous open reading frames (ORFs), whereas magenta squares indicate pseudogenes containing multiple insertions/deletions and splice donor/acceptor site mutations causing preliminary STOP codons. Grey squares highlight incomplete genomic information. Check mark symbols = coding sequences or erosion of SCNN1D was confirmed using transcriptomic data available at the NCBI Sequence Read Archive. ° = No transcriptomic data available. Accession numbers are provided in Supplementary Table 1. There is no transcriptomic data available for Enhydra lutris, but a STOP codon in exon 3 was confirmed in two sub-species, E. lutris kenyoni and E. lutris nereis. The phylogenetic trees and time indices were obtained from Timetree (http://timetree.org). Background colour code: light blue = marine species; green = terrestrial species.