Fig. 2: DB-11-BE87 increases AT1-AT2 differentiation with partial dependency on TGFB pathway activation. | Communications Biology

Fig. 2: DB-11-BE87 increases AT1-AT2 differentiation with partial dependency on TGFB pathway activation.

From: Triggering AHR resolves TGF-β1 induced fibroblast activation and promotes AT1 cell regeneration in alveolar organoids

Fig. 2

A Representative images of live alveolospheres treated at time of plating with increasing doses of LA-13-ZS70. Scale = 500 µM. B Quantification of concentration-response curve for LA-13-ZS70 on GFP adjusted count spheroids. EC50 = 376 nM, R2 = 0.81. n = 4 for all concentration except untreated well, n = 96 for untreated well. Individual data points plotted. C Chemical structure of LA-13-ZS70. D Representative images of live alveolospheres treated at time of plating with increasing doses of DB-11-BE87. Scale = 500 µM. E Quantification of concentration-response curve for DB-11-BE87 on GFP adjusted count spheroids. EC50 = 151 nM, R2 = 0.50. n = 4 for all concentration except untreated well, n = 96 for untreated well. Individual data points plotted. F Chemical structure of DB-11-BE87. G RASL-seq of alveolospheres treated at time of plating with 10 µM LA-13-ZS70. Dotted line indicated ± gene fold change of 2 and p-adjusted value of 0.05 compared to DMSO control. Purple = AT1 genes; Blue = AT2 genes; Green = Airway genes; Yellow = fibrosis genes; Red =  Immune genes; Gray = other genes. n = 4. H RASL-seq of alveolospheres treated at time of plating with 10 µM DB-11-BE87. Dotted line indicated ± gene fold change of 2 and p-adjusted value of 0.05 compared to DMSO control. Purple = AT1 genes; Blue = AT2 genes; Green = Airway genes; Yellow = fibrosis genes; Red = Immune genes; Gray = other genes. n = 4. I Quantification of concentration-response curve for DB-11-BE87 on Hopx expression. EC50 = 62 nM, R2 = 0.69. n = 3–5 for all concentration except untreated well, n = 129 for untreated well. Individual data points plotted. J Quantification of concentration-response curve for DB-11-BE87 on GFP adjusted count spheroids in culture with or without exogenous TGF-β1. + TGF-β1 EC50 = 10.5 pM, R2 = 0.70. − TGF-β1 EC50 = 6.3 pM, R2 = 0.65. n = 6 for all concentration except untreated well, n = 24 for untreated wells. Individual data points plotted.

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