Fig. 5: Functional assays of the IGSF22S132F genotype. | Communications Biology

Fig. 5: Functional assays of the IGSF22S132F genotype.

From: The Vulpes vulpes montana genome provides insights into high-altitude adaptation mechanisms of the Vulpes species

Fig. 5

A Western-blotting analysis of the HEK293T cell lysates transfected with the plasmid of WT, S132F, and empty vector. B The mRNA expression levels of the WT and IGSF22S132F measured by RT-qPCR. C Effect of hypoxic stimulus on the cell viability of HEK293T cells. D Effect of hypoxic stimulus on the apoptosis of HEK293T cells. E Gene expression of the hypoxia-related genes, including AKT1, ABCG2, and VEGFA in transfected HEK293T cells.

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