Fig. 2: Sepsis induces long-lasting changes to the mitochondrial Ca2+ uniporter (MCU) complex. | Communications Biology

Fig. 2: Sepsis induces long-lasting changes to the mitochondrial Ca2+ uniporter (MCU) complex.

From: Sepsis restructures the mitochondrial calcium uniporter complex in the lymphoid tissues of mice and humans

Fig. 2

A–F CLP 30 days. C57Bl/6 mice underwent Sham or CLP and best clinical practices. At 30 days, mice were euthanized, spleen and bone marrow tissues were harvested, and total cell protein lysate isolated and analyzed by immunoblot for MCUR1, MICU1-3, EMRE, MCU, and TOM20. Sham (n = 8 mice) CLP (n = 15 mice) G–I Endotoxemia. C57Bl/6 mice were injected with a single dose of LPS (4 mg/kg; i.p.). At 30 days, spleen and bone marrow tissues were harvested and analyzed for MCUR1, MICU1, MCU, and TOM20. Sham (n = 8 mice) CLP (n = 15 mice) J Mitochondrial protein. Spleen were harvested, and a mitochondria-enriched fraction was isolated and analyzed by immunoblot for MICU1, MCU, and TOM20. Sham (n = 5 mice) CLP (n = 5 mice) K RT-PCR. Densitometry was performed with NIH Image (J). Wilcoxon rank sum. bar = median.

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